Leica SP8X

Optical Configuration

Scan Head

Hybrid confocal scanner with resonant and galvanometric mirrors

Detector Types

HyD Detectors (hybrid photodetectors), PMT (photomultiplier tubes), Standard PMT

Laser Lines

405nm (diode), 458nm, 488nm, 561nm, 633nm solid-state lasers

Objective Lenses

63x 1.40 NA Oil, 40x 1.30 NA Oil, 25x 0.95 NA Water, 10x and 5x air objectives

Image Acquisition

Spatial Resolution

XY: ~200 nm (lateral), Z: ~700 nm (axial) at 405 nm with 63x 1.40 NA objective

Scan Speed

Up to 8000 Hz line scan rate with resonant scanner

Pinhole

Electronically adjustable, ranging from 0.5-600 μm

Maximum Field of View

512×512 to 2048×2048 pixels

Software & Processing

Control Software

Leica LAS X with Lightning deconvolution

Real-time Processing

HyVolution for improved signal and resolution

Environmental Control

Stage

Motorized XY stage with temperature control option

Focus

Motorized Z-drive with definite focus for live-cell imaging

Funding: NIH-funded via S10 OD016240
Contact: Request training or schedule time

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