Guides, protocols, software resources, and training materials for facility users
Whether you are new to the facility or looking to expand your imaging repertoire, these materials are a starting point. Contact us for instrument-specific documentation or to request a custom protocol.
Introduction to light microscopy principles, optical sectioning, and fluorescence. Recommended reading before a first training session.
Fixation, staining, and mounting protocols for common biological samples. Includes recommendations for each instrument in the facility.
Step-by-step setup guides for the Leica SP8X, Zeiss 880, Leica Widefield, and IMARIS workstation — including acquisition parameter templates.
Resources on FRAP, FRET, spectral unmixing, live-cell timelapse, and mosaic tiling strategies.
Tutorials for IMARIS (3D/4D), Fiji/ImageJ, and common analysis workflows including segmentation, particle tracking, and co-localization.
File naming conventions, metadata standards, directory structure recommendations, and archival guidance for microscopy datasets.